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Title   인체 간세포암종 , 간아세포암종 세포조의 성장에 영향을 주는 인자에 관한 연구 ( Study on the Effect of Various Factors for the Growth of Human Hepatocellular Carcinoma , Hepatoblastoma Cell Lines )
Publicationinfo   1991 Jan; 023(01): 83-101.
Key_word   Human hepatocellular carcinoma cell lines, Hepatotrophic factors, Steroid hormones, Growth effects
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Abstract   It is well known that hepatotrophic factors, such as insulin, glucagon, epidermal growth factor, has proliferative effect on hepatocyte and animal hepatocellular carcinoma cell lines. In order to evaluate the effects of insulin, glucagon, epidermal growth factor on the growth of human hepatocellular carcinoma and human hepatoblastoma cell lines, the growth of hepatocellular carcinoma cell lines (Hep G2, SK Hepl, SNU-368, SNU-398) and hepatoblastoma cell line (Hep 3B2) was measured uaing MTT assay (tetraxolium based colorimetric assay). This study also tried to evaluate the growth effects of steroid hormones like estrogen, progesterone, androgen, and hydrocortisone, and the growth effects of growth factors like selenium, triiodothyronine, human grawth hormone, transferrin, ethanolamine/phosphoryl ethanolamine, which are known ta play an essential role in the growth of various cell lines in culture. As a result, insulin enhanced the growth of Hep G2 and SNU-368 cell lines'and glucagon enhanced the growth of Hep G2 cell line, but epidermsl growth factor had no effect on the growth of these cell lines. Estrogen, testosterone, and progesterone had no effect of the growth of these cell lines but hydrocortisone enhanced the growth of SK Hepl cell line. Transferzin enhanced the growth of SK Hepl cell line, selenium enhanced the growth of Hep G2 cell line, and ethanolamine/phosphoryl ethanolamine enhanced the growth af Hep 3B2 cell line. Tbe growth of all hepatocellular carcinoma cell lines was enhanced by insulin-transferrin mixture, but the growth af hepatoblastoma cell line was not enhanced by insulin-transferrin mixture. Theee results revealed that supplementation of insulin-transferrin mixture provided more effective stimulation in the growth of hepatocellular carcinoma cell lines than supplementation of either factor.
저 자   김기덕(Gi Duk Kim),박재갑(Jae Gahb Park),김수태(Soo Tae Kim)